Two-photon confocal laser-scanning microscope

Two-photon excitation as a single quantum event has become a powerful tool for modern biophysical research. Compared with conventional one-photon excitation, it has the advantages of: i) less photo-damage, ii) more contrast, iii) allowing imaging in thicker sections. With this set-up we are performing Ca²⁺ imaging experiments, membrane re-cycling observations and morphological studies.
© 2005 A. W. Gummer. Picture by Dr. C. Harasztosi.